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permeabilization buffer  (R&D Systems)
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Permeabilization Buffer, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow cytometry fixation buffer cat. pf00016  (Proteintech)
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The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow <t>cytometry</t> analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
Flow Cytometry Fixation Buffer Cat. Pf00016, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flow cytometry fixation buffer cat. pf00016/product/Proteintech
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flow cytometry intracellular fixation and permeabilization buffers  (Thermo Fisher)
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The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow <t>cytometry</t> analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
Flow Cytometry Intracellular Fixation And Permeabilization Buffers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow cytometry intracellular fixation and permeabilization buffers - by Bioz Stars, 2026-04
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fixation buffer  (R&D Systems)
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The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow <t>cytometry</t> analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
Fixation Buffer, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x fixation permeabilization buffer  (R&D Systems)
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The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow <t>cytometry</t> analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
1x Fixation Permeabilization Buffer, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1x fixation permeabilization buffer/product/R&D Systems
Average 93 stars, based on 1 article reviews
1x fixation permeabilization buffer - by Bioz Stars, 2026-04
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permeabilization buffer kit  (R&D Systems)
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R&D Systems permeabilization buffer kit
The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow <t>cytometry</t> analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.
Permeabilization Buffer Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/permeabilization buffer kit/product/R&D Systems
Average 93 stars, based on 1 article reviews
permeabilization buffer kit - by Bioz Stars, 2026-04
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flow cytometry fixation permeabilization buffer kit i  (Bio-Techne corporation)
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Bio-Techne corporation flow cytometry fixation permeabilization buffer kit i
Quantification of CHGA + cells in ileum and nasal biopsies as determined by flow <t>cytometry.</t> Percentage of CHGA + cells within the epithelial cell population defined as EpCAM + expression in ileum tissue and nasal tissue from controls, AR patients and CRSwNP patients (n = 5/group). CHGA, chromogranin A; EpCAM: epithelial cellular adhesion molecule; AR, allergic rhinithis; CRSwNP, chronic rhinosinusitis with nasal polyps; data represented as mean ± SD, *** p < 0.005; **** p < 0.001.
Flow Cytometry Fixation Permeabilization Buffer Kit I, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow cytometry fixation & permeabilization buffer kit  (R&D Systems Hematology)
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R&D Systems Hematology flow cytometry fixation & permeabilization buffer kit
Quantification of CHGA + cells in ileum and nasal biopsies as determined by flow <t>cytometry.</t> Percentage of CHGA + cells within the epithelial cell population defined as EpCAM + expression in ileum tissue and nasal tissue from controls, AR patients and CRSwNP patients (n = 5/group). CHGA, chromogranin A; EpCAM: epithelial cellular adhesion molecule; AR, allergic rhinithis; CRSwNP, chronic rhinosinusitis with nasal polyps; data represented as mean ± SD, *** p < 0.005; **** p < 0.001.
Flow Cytometry Fixation & Permeabilization Buffer Kit, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flow cytometry fixation & permeabilization buffer kit/product/R&D Systems Hematology
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The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.

Journal: Journal of Inflammation Research

Article Title: Modified Xi-Jiao-Di-Huang Decoction Alleviates Sepsis via Regulating Macrophage Polarization by Inhibiting the PIM2/NF-κB Pathway

doi: 10.2147/JIR.S509734

Figure Lengend Snippet: The effect of MXJDH on macrophage polarization and Pim2/NF-κB pathway. ( A ) Flow cytometry analysis of the effect of MXJDH on CD86 and CD206 expression in LPS-treated macrophages. ( B ) Co-IP analysis of the formation of Pim2-p65. ( C and D ) Effect of MXJDH and Dexamethasone on inflammatory cytokines TNF-α and IL-10 in LPS-treated macrophages (n=3). ( E ) Protein expression level of p65, Pim2, IL-6, IL-10 and TNF-α. ( F – J ) Statistical analysis of p65, Pim2, IL-6, IL-10 and TNF-α protein levels (n=3). Data were shown as mean ± SD and were analyzed using one-way ANOVA (nonparametric) analysis. *P < 0.05, **p <0.01, ***P < 0.001, VS model group.

Article Snippet: Flow cytometry fixation buffer (Cat. PF00016), and Flow Cytometry Perm Buffer (Cat. PF00017) were purchased from Proteintech (Hubei, China).

Techniques: Flow Cytometry, Expressing, Co-Immunoprecipitation Assay

Quantification of CHGA + cells in ileum and nasal biopsies as determined by flow cytometry. Percentage of CHGA + cells within the epithelial cell population defined as EpCAM + expression in ileum tissue and nasal tissue from controls, AR patients and CRSwNP patients (n = 5/group). CHGA, chromogranin A; EpCAM: epithelial cellular adhesion molecule; AR, allergic rhinithis; CRSwNP, chronic rhinosinusitis with nasal polyps; data represented as mean ± SD, *** p < 0.005; **** p < 0.001.

Journal: Frontiers in Immunology

Article Title: Rare presence and function of neuroendocrine cells in the nasal mucosa

doi: 10.3389/fimmu.2024.1394539

Figure Lengend Snippet: Quantification of CHGA + cells in ileum and nasal biopsies as determined by flow cytometry. Percentage of CHGA + cells within the epithelial cell population defined as EpCAM + expression in ileum tissue and nasal tissue from controls, AR patients and CRSwNP patients (n = 5/group). CHGA, chromogranin A; EpCAM: epithelial cellular adhesion molecule; AR, allergic rhinithis; CRSwNP, chronic rhinosinusitis with nasal polyps; data represented as mean ± SD, *** p < 0.005; **** p < 0.001.

Article Snippet: Sequentially, intracellular staining was performed using Flow Cytometry Fixation & permeabilization Buffer Kit I (FC009, Biotechne) with 50 µl/ml PE Anti-Human Chromogranin A Clone S21-537 (RUO) antibody (5564563, BD Pharmingen).

Techniques: Flow Cytometry, Expressing